絡石藤與地瓜藤抑制NO活性及差異性化學成分研究

黃梓泓¹, 宋鴻志¹, Edward J. Kennelly², 譚欽剛^1,2*

1. 桂林醫學院, 中國廣西桂林 541199;2. 紐約城市大學, 美國紐約 10468

摘要:

絡石藤為藥典記載的常用中藥,而地瓜藤在民間部分地區用作絡石藤。為明確兩種藥材抗炎活性及相關化學成分間的差異,初步探討其可能的藥效物質基礎,該研究通過檢測不同濃度絡石藤與地瓜藤醇提物對RAW264.7細胞存活率的影響,采用Griess試劑法測定LPS誘導的RAW264.7細胞上清液NO釋放量,利用UPLC-Q-TOF-MS/MS技術對絡石藤與地瓜藤醇提物中的化學成分進行分析,并結合多元統計學中的OPLS-DA模型鑒定兩種藥材的差異性化學成分。結果表明:(1)兩種藥材皆具有一定的NO抑制作用,絡石藤粗提物的抑制作用稍強于地瓜藤。(2)絡石藤與地瓜藤的化學成分存在明顯差異,絡石藤中的21個差異性成分主要為木脂素類,而地瓜藤中的10個差異性成分主要為黃酮類。綜上認為,差異性化學成分可能是造成這兩種藥材抗炎活性差異的主要活性物質。該研究結果為絡石藤與地瓜藤的抗炎藥效物質基礎和質量控制提供了理論依據。

關鍵詞: 絡石藤, 地瓜藤, NO抑制, UPLC-Q-TOF-MS/MS, 差異性成分

DOI：10.11931/guihaia.gxzw202209030

分類號:Q946

文章編號:1000-3142(2023)01-0174-09

基金項目:國家自然科學基金(82060783); 廣西自然科學基金(2020GXNSFAA259001)。

Inhibition of NO activity and differential chemical constituents of the medicinal parts in Trachelospermum jasminoides and Ficus tikoua

HUANG Zihong¹, SONG Hongzhi¹, Edward J. Kennelly², TAN Qingang^1,2*

1. Guilin Medical University, Guilin 541199, Guangxi, China;2. City University of New York, New York 10468, USA

Abstract:

Trachelospermum jaminoides, a liana of family Apocynaceae, is used as classic traditional Chinese medicine recorded in the Chinese Pharmacopoeia, and Ficus tikoua, a liana of family Moraceae, is used as its customary supply in some ethnic districts, both of which are used as remedies for the inflammation-related disease. In order to determine the differences of anti-inflammatory activity and related chemical constituents between the two medicinal materials, the cell viability test was used to detect the cytotoxicity(RAW264.7)of the medicinal parts in T. jaminoides and F. tikoua at different concentrations, and the Griess method was applied to detect the inhibitory effects of the two species on nitric oxide(NO)production in LPS-induced RAW264.7 cells in vitro. The ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS)combined with the OPLS-DA model in multivariate statistics were used to identify the differential chemical constituents between the medicinab parts in T. jaminoides and F. tikoua. The results were as follows:(1)The NO production inhibition rate of T. jaminoides is stronger than that of F. tikoua.(2)Twenty-one differential chemical components were identified in the stems with leaves of T. jaminoides, most of which were lignans. Ten differential chemical components in the aerial parts of F. tikoua, most flavonoids, were identified. In summary, the differential chemical constituents may be the main active substances that cause the difference in anti-inflammatory activity between the two medicinal materials. This study provides a theoretical basis for the anti-inflammatory medicinal material basis and quality control of the medicinal parts in T. jaminoides and F. tikoua.

Key words: Trachelospermum jaminoides, Ficus tikoua, NO inhibition, UPLC-Q-TOF-MS/MS, differential chemical constituents