蒙古蒼耳抑制壞死性凋亡(necroptosis)活性部位化學成分研究(Ⅰ)

盛天露¹, 付璐鷺¹, 許俊申¹, 尚銳峰¹, 陳鐘文¹, 劉峰², 劉華^1*

1. 江西中醫藥大學藥學院, 南昌 330004;2. 江西中醫藥大學附屬醫院, 南昌 330006

摘要:

蒼耳(Xanthium strumarium)為民間傳統藥用植物,本課題組前期研究發現蒙古蒼耳大孔樹脂50%乙醇洗脫部位具有顯著的抑制細胞壞死性凋亡(necroptosis)活性。為明確蒙古蒼耳(X. mongolicum)的活性成分,活性部位采用反復硅膠柱色譜、Sephadex LH-20柱色譜、制備高效液相色譜、重結晶等多種方法進行分離和純化,運用NMR、MS等波譜方法并結合文獻數據對分離得到的化合物進行結構鑒定。結果表明:從該活性部位中分離得到14個化合物,分別鑒定為hydroxydihydrobovolide(1)、樹莓酮(2)、水楊醇(3)、對羥基苯乙酮(4)、對羥基苯甲醛(5)、咖啡酸乙酯(6)、阿魏醛(7)、異東莨菪素(8)、3,3'-bis(3,4-dihydro-4-hydroxy-6-methoxy-2H-1-benzopyran)(9)、axillarin(10)、槲皮素(11)、(+)松脂素(12)、β-谷甾醇(13)和棕櫚酸(14)。化合物1-4、7-10均為首次從蒼耳中分離得到。

關鍵詞: 蒼耳, 壞死性凋亡, 活性部位, 化學成分, 江西

DOI：10.11931/guihaia.gxzw202108047

分類號:Q946

文章編號:1000-3142(2023)01-0132-07

基金項目:國家自然科學基金(81760706); 江西省中醫藥大學中藥學一流學科專項科研基金(JXSYLXK-ZHYAO022,JXSYLXK-ZHYAO112)。

Chemical constituents of the necroptosis-inhibited active fraction from Xanthium mongolicum(Ⅰ)

SHENG Tianlu¹, FU Lulu¹, XU Junshen¹, SHANG Ruifeng¹, CHEN Zhongwen¹, LIU Feng², LIU Hua^1*

1. College of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang 330004, China;2. Affiliated Hospital, Jiangxi University of Chinese Medicine, Nanchang 330006, China

Abstract:

Xanthium strumarium is a traditional folk medicinal plant. The 50% ethanol eluting faction of X. mongolicum had significant activity to inhibit necroptosis. In order to investigate the active constituents, the active fraction of X. mongolicum were isolated and purified by various chromatographic methods such as repeated silica gel column chromatography, Sephadex LH-20 column chromatography, preparative high performance liquid chromatography and recrystallization. Their structures were established on the basis of NMR, MS spectraoscopic analyses and comparison with lirerature date. The results were as follows: A total of 14 compounds were isolated from the active part and identified as hydroxydihydrobovolide(1), raspberry ketone(2), salicyl alcohol(3), 4-hydroxyl-acetophenone(4), 4-hydroxybenzaldehyde(5), ethyl caffeate(6), ferulaldehyde(7), isoscopletin(8), 3,3'-bis(3,4-dihydro-4-hydroxy-6-methoxy-2H-1-benzopyran)(9), axillarin(10), quercetin(11),(+)pinoresinol(12), β-sitosterol(13)and palmatic acid(14). Compounds 1-4, 7-10 were all isolated from Xanthium mongolicum for the first time.

Key words: Xanthium mongolicum, necroptosis, active fraction, chemical constituents, Jiangxi